Identification of Unambiguous Borrelia Peptides in Human Urine Using Affinity Capture and Mass Spectrometry
Rocio Cornero1, Sumaiya Safia Irfan1, Silvia Cachaco1
1Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|January 2, 2024
Abstract
None:
The combination of advanced mass spectrometry and enrichment-based sample preparation methods has enhanced analytical capabilities in clinical proteomics. In this chapter, we describe a method of proteome analysis to identify Borrelia-derived peptides in urine that includes a sample affinity enrichment method coupled with liquid chromatography tandem mass spectrometry analysis and a bioinformatic peptide authentication algorithm.


