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Related Experiment Video

Updated: Jul 14, 2026

Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

Temporal Changes in Innate and Adaptive Immunity During Sepsis as Determined by ELISpot.

J Unsinger, D Osborne, A H Walton

Biorxiv : the Preprint Server for Biology

|January 3, 2024

Summary

The ELISpot assay quantifies immune cell function in sepsis, detecting changes in innate and adaptive immunity. This assay can monitor immune responses to therapies, aiding sepsis treatment development.

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A Data-Driven Approach to Quantifying Immune States in Sepsis

A Data-Driven Approach to Quantifying Immune States in Sepsis

Published on: February 7, 2025

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Last Updated: Jul 14, 2026

Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Enzyme-linked Immunospot Assay ELISPOT: Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens

Published on: November 23, 2010

Design of Cecal Ligation and Puncture and Intranasal Infection Dual Model of Sepsis-Induced Immunosuppression

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Published on: June 15, 2019

A Data-Driven Approach to Quantifying Immune States in Sepsis

A Data-Driven Approach to Quantifying Immune States in Sepsis

Published on: February 7, 2025

Area of Science:

Immunology
Sepsis Research
Assay Development

Background:

Sepsis hinders novel immune therapy development due to challenges in rapid, quantitative host immunity evaluation.
The Enzyme-Linked ImmunoSpot (ELISpot) assay measures cytokine secretion at the single-cell level, offering a broad dynamic range for precise immune assessment.
This study investigates ELISpot's ability to track dynamic changes in innate and adaptive immunity during sepsis and assess immune-modulating drug effects.

Approach:

Sublethal cecal ligation and puncture (CLP) model induced sepsis in mice.
Blood and spleen samples were collected serially for ex vivo interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) analysis using ELISpot and ELISA.
The impact of in vivo immune therapies (dexamethasone, IL-7, arginine) on innate and adaptive immunity was evaluated using ELISpot.

Key Points:

ELISpot confirmed reduced innate and adaptive immune responsiveness during sepsis progression.
The assay detected quantifiable changes in immune responses to dexamethasone, arginine, and IL-7, aligning with their known mechanisms of action.
ELISpot and ELISA results showed parallel trends, with some notable differences.

Conclusions:

ELISpot provides a unique method for assessing the functional status of both adaptive and innate immunity over time.
The study demonstrates ELISpot's utility in detecting and monitoring in vivo drug effects on sepsis-induced immune dysfunction.
This capability represents a significant advancement for guiding novel immune therapies in sepsis management.