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Researchers developed a new genetically encoded sensor (GEMS) to track N6-methyladenosine (m6A) RNA methylation in real-time within living cells, aiding drug discovery.

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Biotechnology

Background:

  • N6-methyladenosine (m6A) is a prevalent mRNA modification regulating gene expression and cellular functions.
  • Dysregulation of m6A is implicated in various human diseases, making m6A methyltransferases potential therapeutic targets.
  • Existing methods for m6A detection require RNA isolation and lack real-time monitoring in live cells.

Purpose of the Study:

  • To develop a novel technology for real-time sensing of m6A RNA methylation in living cells.
  • To create a versatile platform for m6A detection and m6A-dependent protein delivery.

Main Methods:

  • Development of a genetically encoded m6A sensor (GEMS) that links fluorescence to cellular mRNA methylation.
  • Utilizing GEMS to monitor m6A changes in response to pharmacological inhibition of m6A methyltransferases.

Main Results:

  • GEMS successfully provides a real-time readout of mRNA methylation levels in living cells.
  • The sensor detects m6A alterations induced by methyltransferase inhibitors, demonstrating utility in drug discovery.
  • GEMS can be engineered for m6A-dependent delivery of protein payloads.

Conclusions:

  • GEMS technology offers a versatile platform for m6A sensing with a simple readout.
  • This platform has potential applications in drug discovery and targeted protein delivery based on m6A levels.