Patterns of crystallin expression during differentiation in vitro of several chick genotypes with different effects...

We have recently reported that chick lens cells during differentiation in long-term culture show a programme of change in crystallin expression which mimics events during lens development in vivo. The aim of the present work was to examine the stability of the programme by testing the response to genetic influences and exposure to a carcinogen. Five genetically distinct inbred strains of chick, differing in the intrinsic growth rates of lens epithelial cells in vitro, were used to study the effects of the rate of mitosis on crystallin expression, both during lens development and in long-term cell culture. The time of appearance of lentoids, their size and abundance and the rate of change in crystallin expression were all modified in a genotype-specific way, related to the rate of mitosis, but the programme of changes in crystallin expression was the same for all genotypes. Genetic differences were also found in the patterns of response to treatment of cultures during the logarithmic growth phase with a nitrosoguanidine compound known to affect cell differentiation in lens cultures and several other systems. The changes in crystallin expression and fibre differentiation were delayed, but cultures of the faster growing genotypes were least affected. With further culture, crystallin expression tended to recover to control values although levels of fibre differentiation and cell growth remained depressed. The results indicate that genetic differences in intrinsic growth rate moderate but do not change the programme of crystallin expression shown by lens epithelial cells in culture, and that this programme shows resistance to change.