Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Left to dry: unseen risks lurking on reusable medical devices.

Scientific reports·2025

Same author

Efficacy of cleaning, disinfection, and sterilization modalities for addressing infectious drug-resistant fungi: a review.

Journal of applied microbiology·2025

Same author

Affinity and Inactivation of Bacterial Endotoxins for Medical Device Materials.

Biomedical instrumentation & technology·2024

Same author

Validation of the Device Feature Approach for Reusable Medical Device Cleaning Evaluations.

Biomedical instrumentation & technology·2024

Same author

Test Soil and Material Affinity for Reusable Device Cleaning Validations.

Biomedical instrumentation & technology·2024

Same author

Cleaning Challenges: Can Extended Soil Dry Times Be Reversed?

Biomedical instrumentation & technology·2023

Search research articles

Related Experiment Video

Updated: Jul 6, 2025

Failure of Cleaning Verification in Pharmaceutical Industry Due to Uncleanliness of Stainless Steel Surface

Failure of Cleaning Verification in Pharmaceutical Industry Due to Uncleanliness of Stainless Steel Surface

Published on: August 11, 2017

Improving Protein Assay Methods to More Accurately Assess Medical Device Cleanliness.

Terra A Kremer, Allan Kimble, Christopher Ratanski

Biomedical Instrumentation & Technology

|January 3, 2024

Summary

This summary is machine-generated.

Standard protein assays struggle to accurately detect low protein levels on medical devices. Method improvements for the micro-bicinchoninic acid assay enhance sensitivity and accuracy for reliable cleaning validation.

More Related Videos

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

Related Experiment Videos

Last Updated: Jul 6, 2025

Failure of Cleaning Verification in Pharmaceutical Industry Due to Uncleanliness of Stainless Steel Surface

Failure of Cleaning Verification in Pharmaceutical Industry Due to Uncleanliness of Stainless Steel Surface

Published on: August 11, 2017

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Modified Most Probable Number Assay to Quantify Salmonella in Raw and Ready-to-Cook Chicken Products

Published on: January 31, 2025

Area of Science:

Biochemistry
Analytical Chemistry
Medical Device Reprocessing

Background:

Current protein assays for reusable medical devices lack sensitivity for low residual protein concentrations.
Standard curves in existing methods show sensitivity issues at critical protein levels (6.4 μg/cm²).

Purpose of the Study:

To improve the sensitivity and accuracy of the micro-bicinchoninic acid assay for detecting protein residuals.
To enhance the reliability of protein detection for reusable medical device cleaning validation.

Main Methods:

Analytical validation criteria were applied to modify the micro-bicinchoninic acid assay.
Method enhancements included incorporating a standard addition method, increasing well volume, and altering the working reagent ratio.

Main Results:

The improved micro-bicinchoninic acid assay demonstrated increased sensitivity and accuracy.
Reliable detection of low protein levels relevant to device cleaning validation was achieved.

Conclusions:

Enhanced micro-bicinchoninic acid assay methods provide more accurate protein residual detection.
These improvements are crucial for validating the cleanliness of reusable medical devices.