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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Jul 6, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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SYNCAS: Efficient CRISPR/Cas9 gene-editing in difficult to transform arthropods.

Sander De Rouck1, Antonio Mocchetti1, Wannes Dermauw1

  • 1Laboratory of Agrozoology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Belgium.

Insect Biochemistry and Molecular Biology
|January 3, 2024
PubMed
Summary

Researchers optimized CRISPR/Cas9 delivery for arthropod genome editing using a novel formulation called SYNCAS. This method significantly boosts gene knock-out efficiency in species like the two-spotted spider mite and western flower thrips.

Keywords:
ABC-transportersBAPCCas9 deliveryChitin synthaseEndosomal escape reagentHox genes

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Area of Science:

  • Genetics
  • Molecular Biology
  • Entomology

Background:

  • CRISPR/Cas9 is a powerful genome editing tool with broad applications in arthropod research.
  • Traditional microinjection methods are inefficient and challenging for many arthropod species, including the two-spotted spider mite (Tetranychus urticae).

Purpose of the Study:

  • To optimize CRISPR/Cas9 delivery for efficient maternal injection in T. urticae.
  • To develop a novel CRISPR/Cas9 formulation for improved genome editing efficiency in hard-to-transform arthropods.

Main Methods:

  • Exploration of various CRISPR/Cas9 formulations for maternal injection in T. urticae.
  • Development and testing of a synergistic formulation combining branched amphipathic peptide capsules and saponins (SYNCAS).
  • Application of SYNCAS for gene knock-outs, co-CRISPR strategies, and knock-in mutations in T. urticae and gene knock-outs in Frankliniella occidentalis.

Main Results:

  • A novel CRISPR/Cas9 formulation, SYNCAS, demonstrated a synergistic effect, significantly increasing knock-out efficiency to over 20% in T. urticae.
  • SYNCAS enabled targeted gene knock-outs (phytoene desaturase, CYP384A1, Antennapedia), co-CRISPR, and knock-in mutant generation in T. urticae.
  • Successful application of SYNCAS for gene knock-outs in the western flower thrips (Frankliniella occidentalis).

Conclusions:

  • The SYNCAS formulation represents a breakthrough in CRISPR/Cas9 delivery for arthropods, overcoming previous limitations.
  • This method facilitates routine genome editing in previously challenging species and holds potential for broader application in arthropod genetic research.