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Related Experiment Video

Updated: Jul 6, 2025

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
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"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

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Microfluidic 3D hepatic cultures integrated with a droplet-based bioanalysis unit.

Jose M de Hoyos-Vega1, Alan M Gonzalez-Suarez1, Diana F Cedillo-Alcantar2

  • 1Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, USA.

Biosensors & Bioelectronics
|January 4, 2024
PubMed
Summary

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This study introduces a microfluidic device for analyzing cell function in tiny volumes without disturbing cultures. It enables precise measurement of glucose and albumin in hepatic spheroids, advancing cell-based research.

Area of Science:

  • Biomedical Engineering
  • Cell Biology
  • Analytical Chemistry

Background:

  • Microfluidic cell culture analysis faces challenges in maintaining culture integrity and local signaling gradients.
  • On-chip analysis of picoliter volumes is crucial for understanding cell function in microenvironments.

Purpose of the Study:

  • To develop an integrated microfluidic device for on-chip bioanalysis of picoliter cell culture media.
  • To enable precise measurement of cellular metabolites and proteins in 3D cell cultures.

Main Methods:

  • Fabrication of a microfluidic device with 140 microwells (300 µm diameter) for 3D spheroid formation.
  • Integration of a droplet generator and micromechanical valves for sequential reagent loading.
  • Enzymatic and immunoassays within 0.8 nL droplets for glucose and albumin detection.
Keywords:
Automated microfluidicsGlucose metabolismHepatic spheroidsIn-droplet assayOn-chip bioanalysis

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Main Results:

  • Demonstrated on-chip analysis of picoliter volumes of conditioned media from hepatic spheroids.
  • Successfully measured glucose consumption and release in response to hormonal stimulation (insulin, glucagon).
  • Validated the device's capability to mimic physiological feeding and fasting states.

Conclusions:

  • The developed microfluidic device facilitates sensitive, on-chip bioanalysis of microscale cell cultures.
  • This technology supports the study of cellular metabolism and hormonal responses in 3D spheroid models.
  • Potential applications include investigating metabolic syndrome, insulin resistance, and diabetes.