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Related Experiment Video

Updated: Jul 6, 2025

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
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HDAC3 Contributes to Ischemic Stroke by Regulating Interferon Pathway.

Jiaxin Wang1,2, Mengmeng Yang1, Yang Chen3

  • 1Department of Anesthesiology, First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, 230001 Hefei, Anhui, China.

Journal of Integrative Neuroscience
|January 4, 2024
PubMed
Summary
This summary is machine-generated.

Histone deacetylase 3 (HDAC3) inhibition ameliorates ischemic stroke injury by downregulating ZBP1/p-IRF3 signaling. This novel mechanism offers a promising therapeutic target for stroke treatment.

Keywords:
Z-DNA binding protein 1histone deacetylase 3ischemic strokephosphorylated Interferon Regulatory Factor 3

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Evaluating Cell Death Signaling by Immunofluorescence in a Rat Model of Ischemic Stroke
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Area of Science:

  • Neuroscience
  • Immunology
  • Molecular Biology

Background:

  • Inflammation and immune responses are key in ischemic stroke pathology.
  • Interferon signaling pathway alterations occur in acute stroke.
  • The role of histone deacetylation in stroke is not well understood.

Purpose of the Study:

  • To investigate if modulating histone deacetylation can regulate the interferon signaling pathway in stroke.
  • To determine if HDAC3 inhibition impacts stroke-related pathological changes.

Main Methods:

  • Middle cerebral artery occlusion (MCAO) mouse model.
  • Treatment with HDAC3 inhibitor RGFP966.
  • Real-time PCR, Western blot, TTC staining, behavioral tests, and confocal imaging.

Main Results:

  • RGFP966 treatment reduced infarct volume and anxiety-like behavior in MCAO mice.
  • RGFP966 inhibited interferon pathway hyperactivation and Z-DNA Binding Protein 1 (ZBP1) expression in microglia.
  • HDAC3 inhibition ameliorated inflammatory responses post-stroke.

Conclusions:

  • HDAC3 inhibition ameliorates ischemic stroke injury via downregulation of the ZBP1/phosphorylated Interferon Regulatory Factor 3 (p-IRF3) pathway.
  • This study reveals a novel therapeutic mechanism for ischemic stroke.
  • Targeting HDAC3 presents a promising strategy for future stroke therapies.