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Substrate utilization by fetal pig skeletal muscle.

D R Campion, R L Wilson

    Journal of Animal Science
    |December 1, 1986
    PubMed
    Summary

    Fetal pig skeletal muscle metabolism of glucose, fructose, lactate, acetate, and palmitate was studied. Lactate oxidation and lipid incorporation varied with fetal age, indicating developmental changes in muscle energy utilization.

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    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Muscle Physiology

    Background:

    • Fetal skeletal muscle development involves significant metabolic adaptations.
    • Understanding substrate utilization is crucial for fetal growth and maturation.

    Purpose of the Study:

    • To investigate the in vitro metabolic capacity of fetal pig skeletal muscle (biceps femoris) for various substrates at different gestational ages.
    • To determine the influence of fetal age and substrate concentration on glucose, fructose, lactate, acetate, and palmitate metabolism.

    Main Methods:

    • In vitro incubation of fetal pig biceps femoris muscle samples from 70, 90, and 110 days of gestation.
    • Measurement of substrate oxidation to CO2, incorporation into glycogen, phospholipids, and triacylglycerols.
    • Assay of succinate dehydrogenase and glucose-6-phosphate dehydrogenase activities.

    Main Results:

    • Succinate dehydrogenase activity increased with fetal age, but oxidation rates of glucose, fructose, acetate, and palmitate were age-independent and dose-dependent.
    • Lactate oxidation was higher in 70-day fetuses compared to older fetuses.
    • Muscle glycogen content and glucose incorporation into glycogen increased with gestational age.
    • Pentose cycle activity was evident, and lactate and palmitate incorporation into phospholipids was greatest at 70 days.

    Conclusions:

    • Fetal pig skeletal muscle exhibits distinct metabolic profiles that change during gestation.
    • Early gestational stages (70 days) show higher lactate utilization and lipid incorporation, coinciding with muscle fiber development.
    • The pentose phosphate pathway is active, contributing to fetal muscle metabolism.

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