"Review of strategic methods for encapsulating essential oils into chitosan nanosystems and their applications"

Essential oils (EOs) are hydrophobic, concentrated extracts of botanical origin containing diverse bioactive molecules that have been used for their biomedical properties. On the other hand, the volatility, toxicity, and hydrophobicity limited their use in their pure form. Therefore, nano-encapsulation of EOs in a biodegradable polymeric platform showed a solution. Chitosan (CS) is a biodegradable polymer that has been intensively used for EOs encapsulation. Various approaches such as homogenization, probe sonication, electrospinning, and 3D printing have been utilized to integrate EOs in CS polymer. Different CS-based platforms were investigated for EOs encapsulation such as nanoparticles (NPs), nanofibers, films, nanoemulsions, 3D printed composites, and hydrogels. Biological applications of encapsulating EOs in CS include antioxidant, antimicrobial, and anticancer functions. This review explores the principles for nanoencapsulation strategies, and the available technologies are also reviewed, in addition to an in-depth overview of the current research and application of nano-encapsulated EOs.