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DNase production by Clostridium septicum.

P J Swiatek, S D Allen, J A Siders

    Journal of Clinical Microbiology
    |February 1, 1987
    PubMed
    Summary

    Clostridium septicum isolates exhibit significant extracellular DNase activity, exceeding other tested clostridial species. This enzyme, with a molecular weight of approximately 45,000, is a major secreted protein.

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    Area of Science:

    • Microbiology
    • Enzymology

    Background:

    • Clostridium septicum is a species of anaerobic bacteria.
    • Extracellular DNases are enzymes that degrade DNA.

    Purpose of the Study:

    • To quantify and characterize the extracellular DNase activity of Clostridium septicum isolates.
    • To compare DNase production in C. septicum with other clostridial species.

    Main Methods:

    • Assay of extracellular DNase activity in 62 C. septicum isolates.
    • Determination of the molecular weight of the C. septicum DNase using biochemical methods.

    Main Results:

    • All tested C. septicum isolates demonstrated significant extracellular DNase activity.
    • C. septicum DNase activity was notably higher than in other tested clostridial isolates.
    • The molecular weight of C. septicum DNase was determined to be approximately 45,000 Daltons.

    Conclusions:

    • Clostridium septicum is a potent producer of extracellular DNase.
    • DNase is identified as a major extracellular protein secreted by C. septicum.
    • This finding may have implications for understanding C. septicum virulence and pathogenesis.

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