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Updated: Jun 10, 2026
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Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples
Published on: June 15, 2012
Isolation of Single Circulating Tumor Cells Using VyCAP Puncher System.
Thais Pereira-Veiga1,2, Bianca Behrens3, Joska J Broekmaat4
1Roche-Chus Joint Unit, Translational Medical Oncology Group, Oncomet, Health Research Institute of Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|January 9, 2024
Summary
Tumor heterogeneity drives cancer evasion and treatment resistance. This study details a protocol for isolating single circulating tumor cells (CTCs) using the VyCAP Puncher system, advancing liquid biopsy analysis.
Area of Science:
- Oncology
- Biotechnology
- Cancer Research
Background:
- Tumor heterogeneity is a key factor in cancer's ability to evade treatments and develop resistance.
- Single-cell isolation technologies are increasingly important for studying solid tumors, surpassing bulk analysis.
- Analyzing circulating tumor cells (CTCs) via single-cell methods offers insights into therapy-resistant CTCs within liquid biopsies.
Purpose of the Study:
- To describe a protocol for isolating single circulating tumor cells (CTCs).
- To address the challenges of analyzing weak and scarce CTCs in liquid biopsies.
- To leverage single-cell analysis for a deeper understanding of tumor heterogeneity.
Main Methods:
- Utilizing the VyCAP Puncher system for cell isolation.
- Focusing on single-cell isolation techniques for CTCs.
- Developing a protocol applicable to liquid biopsy analysis.
Main Results:
- A protocol for single-cell level isolation of CTCs is presented.
- The method facilitates the study of CTC heterogeneity.
- Addresses limitations in current CTC analysis.
Conclusions:
- Single-cell isolation of CTCs is crucial for understanding tumor heterogeneity and treatment resistance.
- The VyCAP Puncher system offers a viable method for CTC isolation.
- This protocol advances the field of liquid biopsy and cancer research.
