Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography.

Journal of visualized experiments : JoVE·2016
Same author

MiR-15a contributes abnormal immune response in myasthenia gravis by targeting CXCL10.

Clinical immunology (Orlando, Fla.)·2016
Same author

Minicells, Back in Fashion.

Journal of bacteriology·2016
Same author

A new variant of rabbit hemorrhagic disease virus G2-like strain isolated in China.

Virus research·2016
Same author

Tumour-suppressive role of PTPN13 in hepatocellular carcinoma and its clinical significance.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine·2016
Same author

Gonyautoxin 1/4 aptamers with high-affinity and high-specificity: From efficient selection to aptasensor application.

Biosensors & bioelectronics·2016
Same journal

Beyond strain-specific immunity: Conserved antigenic targets, emerging platforms, and translational challenges in universal influenza and pan-coronavirus vaccine development.

Journal of virological methods·2026
Same journal

Analytical characteristics of the NeuMoDx™ SARS-CoV-2 assay and clinical agreement with the BD MAX system.

Journal of virological methods·2026
Same journal

Development of a real-time PCR assay for tracking the spatiotemporal dynamics of a cyanophage and its Synechococcus host in waters of shrimp aquaculture ecosystems.

Journal of virological methods·2026
Same journal

A universal RdRp-targeted primer set for broad, non-specific detection of Quinvirinae and other Betaflexiviridae infecting stone fruits.

Journal of virological methods·2026
Same journal

Development of a multiplex real-time PCR method for detecting immunosuppressive viruses and its preliminary application in broilers.

Journal of virological methods·2026
Same journal

Development of a quantum dot-based immunochromatographic test strip for rapid detection of infectious bursal disease virus.

Journal of virological methods·2026
See all related articles

Related Experiment Video

Updated: Jul 6, 2025

Optimization of a Quantitative Micro-neutralization Assay
10:09

Optimization of a Quantitative Micro-neutralization Assay

Published on: December 14, 2016

21.2K

A method for screening CDV microneutralization activity in microvolume samples.

Xiaoyu Deng1, Jiazi Su2, Bo Hu1

  • 1Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun 130112, China.

Journal of Virological Methods
|January 9, 2024
PubMed
Summary
This summary is machine-generated.

A new screening method for canine distemper virus (CDV) microneutralizing activity was developed using Indirect Immunofluorescence Assay (IFA). This economical and stable method is suitable for microvolume samples and aids in developing CDV neutralizing antibodies.

Keywords:
CDV microneutralizing activityMicroneutralizing activity screening methodMicrovolume samplesNeutralizing antibody

More Related Videos

Measuring Influenza Neutralizing Antibody Responses to AH3N2 Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
12:09

Measuring Influenza Neutralizing Antibody Responses to AH3N2 Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells

Published on: November 22, 2017

18.1K
A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay
05:53

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

Published on: December 7, 2021

4.6K

Related Experiment Videos

Last Updated: Jul 6, 2025

Optimization of a Quantitative Micro-neutralization Assay
10:09

Optimization of a Quantitative Micro-neutralization Assay

Published on: December 14, 2016

21.2K
Measuring Influenza Neutralizing Antibody Responses to AH3N2 Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
12:09

Measuring Influenza Neutralizing Antibody Responses to AH3N2 Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells

Published on: November 22, 2017

18.1K
A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay
05:53

A Step-By-Step Method to Detect Neutralizing Antibodies Against AAV using a Colorimetric Cell-Based Assay

Published on: December 7, 2021

4.6K

Area of Science:

  • Veterinary Virology
  • Immunology

Background:

  • Canine distemper virus (CDV) poses a significant threat to canine health.
  • Accurate and efficient methods for assessing neutralizing antibody responses are crucial for vaccine development and disease control.

Purpose of the Study:

  • To establish a microvolume screening method for canine distemper virus (CDV) microneutralizing activity.
  • To develop a high-throughput assay for detecting CDV neutralizing antibodies.

Main Methods:

  • The method utilizes Indirect Immunofluorescence Assay (IFA) on Vero-slam cells.
  • A CDV nucleoprotein (NP) monoclonal antibody was selected for assay sensitivity.
  • The minimum stable infection concentration of CDV was determined to establish the neutralizing solution.

Main Results:

  • The lowest detection limit for microneutralizing activity was achieved with a CDV neutralizing titer of 1:708 diluted 2^12 times.
  • The detection rate of microneutralizing activity at this limit was 63.54 ± 4.774%.

Conclusions:

  • An economical, stable, and user-friendly high-throughput screening method for CDV microneutralizing activity was successfully established.
  • This method provides a foundation for developing canine CDV neutralizing antibodies from single B cells.