Related Experiment Video
Updated: Jul 6, 2025
07:48
A Method for Lineage Tracing of Corneal Cells Using Multi-color Fluorescent Reporter Mice
Published on: December 18, 2015
17.4K
DNA Barcoding Technology for Lineage Recording and Tracing to Resolve Cell Fate Determination
1Department of Microbiology, Gachon University College of Medicine, Incheon 21999, Republic of Korea.
Cells
|January 11, 2024
Summary
New molecular recording technologies enable detailed cell lineage tracing, revealing developmental and disease processes. This helps understand cellular adaptation and transformations in complex biological systems.
Area of Science:
- Cellular Biology
- Genomics
- Biotechnology
Background:
- Cellular state transitions are fundamental to development, tissue maintenance, immune responses, and disease pathogenesis.
- Understanding cell lineage commitment and adaptation is crucial for both homeostasis and disease progression.
- DNA-based molecular barcoding offers insights into cell lineage history and timing.
Purpose of the Study:
- To comprehensively review emerging molecular recording technologies for cell lineage tracing.
- To detail the principles, applications, and constraints of these advanced technologies.
- To enable comprehensive lineage tracing of all cells within complex biological systems.
Main Methods:
- Utilizing CRISPR/Cas systems for molecular recording.
- Employing base editing for precise genomic alterations.
- Leveraging recombination-based systems and innate variable sequences.
- Documenting phylogenetic trees and deterministic cell lineage events.
Main Results:
- Emerging technologies provide unprecedented resolution for tracking cell lineages.
- These methods allow detailed documentation of cell state transitions and developmental pathways.
- The review synthesizes recent advancements in genome-based molecular recording.
Conclusions:
- Advanced molecular recording technologies are revolutionizing cell lineage tracing.
- These tools are essential for understanding complex biological processes like development and disease.
- Future applications promise comprehensive mapping of cellular histories in vivo.
Related Concept Videos
02:54Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K
01:21Lineage Commitment
3.0K
Commitment is the process whereby stem cells:
3.0K
03:00Next-generation Sequencing
88.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.9K