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Related Concept Videos

Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
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Related Experiment Video

Updated: Jul 5, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
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Wnt3a Facilitates SARS-CoV-2 Pseudovirus Entry into Cells.

Ivonne Melano1, Hui-Jye Chen1, Loveness Ngwira2

  • 1Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404, Taiwan.

International Journal of Molecular Sciences
|January 11, 2024
PubMed
Summary

Wnt3a facilitates SARS-CoV-2 entry by activating Wnt/β-catenin signaling, which increases ACE2 transcription. This finding is crucial for developing new therapies against coronavirus infections.

Keywords:
ACE2SARS-CoV-2Wnt3acanonical pathwayhost factorvirus entryβ-catenin

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Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • The primary host receptor for SARS-CoV-2, ACE2, has low lung expression, posing a puzzle for its widespread infection.
  • Understanding SARS-CoV-2 infection mechanisms is vital for developing effective therapeutic strategies against coronaviruses.

Purpose of the Study:

  • To investigate the role of Wnt3a, a previously identified potential host factor, in SARS-CoV-2 entry.
  • To elucidate the molecular mechanisms by which Wnt3a influences SARS-CoV-2 infection and ACE2 expression.

Main Methods:

  • Validated Wnt3a's significance using shRNA-mediated knockdown and overexpression experiments.
  • Assessed SARS-CoV-2 pseudotyped virus entry into cells.
  • Analyzed the activation of the Wnt/β-catenin signaling pathway and its effect on ACE2 transcription.

Main Results:

  • Wnt3a was confirmed as a significant host factor for SARS-CoV-2 entry.
  • SARS-CoV-2 pseudotyped virus infection activates the Wnt/β-catenin signaling pathway.
  • Activated Wnt/β-catenin signaling stimulates ACE2 transcription, facilitating virus entry.

Conclusions:

  • Wnt3a plays a critical role in facilitating ACE2-mediated SARS-CoV-2 infection.
  • The Wnt/β-catenin pathway is a key mediator linking Wnt3a to increased ACE2 expression and virus entry.
  • This research provides insights into viral entry mechanisms, aiding the development of novel coronavirus therapeutics.