Related Experiment Video
Updated: Jul 5, 2025
12:52
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
15.8K
TENT5A Increases Glioma Malignancy and Promotes its Progression
Jiali Hu1,2, Lei Zeng1,2, Ronghuan Hu1,2
1Department of Oncology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
Recent Patents on Anti-Cancer Drug Discovery
|January 11, 2024
Summary
Terminal nucleotidyltransferase 5A (TENT5A) is highly expressed in glioma and linked to poorer outcomes. Reducing TENT5A levels inhibits glioma cell growth and spread, suggesting TENT5A as a potential therapeutic target.
Area of Science:
- Neuro-oncology
- Molecular Biology
- Cancer Research
Background:
- Terminal nucleotidyltransferase 5A (TENT5A) is increasingly recognized for its high expression in glioblastoma.
- Elevated TENT5A levels correlate with a poor prognosis in glioblastoma patients.
Purpose of the Study:
- To precisely determine TENT5A expression across different glioma grades.
- To elucidate the functional role of TENT5A in glioma progression and development.
Main Methods:
- Bioinformatic analysis utilizing GEPIA online tools.
- Validation through qRT-PCR, Western blot, and Immunohistochemistry in glioma samples.
- Functional assays including CCK8, colony formation, Transwell, flow cytometry, and scratch assays.
Main Results:
- TENT5A expression is significantly elevated in glioma tissues and positively associated with higher pathological grades.
- Downregulation of TENT5A markedly inhibited glioma cell proliferation, colony formation, invasion, and migration.
- Conversely, overexpression of TENT5A demonstrated lethal effects on glioma cells.
Conclusions:
- TENT5A expression levels are indicative of glioma pathological grade.
- TENT5A plays a critical role in promoting glioma cell proliferation, invasion, and migration.
- Targeting TENT5A presents a promising new therapeutic strategy for glioma treatment.
Related Concept Videos
02:07Tumor Progression
6.3K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.3K
03:03mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K
02:53Adaptive Mechanisms in Cancer Cells
5.8K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.8K
02:18Cancer
48.5K
Cancers arise due to mutations in genes involved in the regulation of cell division, which leads to unrestricted cell proliferation. Modern science and medicine have made great strides in the understanding and treatment of cancer, including eradicating cancer in some patients. However, there is still no cure for cancer. This is largely due to the fact that cancer is a large group of many diseases.
48.5K
01:33Cancer-Critical Genes I: Proto-oncogenes
8.9K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.9K
02:17The Tumor Microenvironment
6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K