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Abstract:
A roller pump is described which is able to pump microlitre quantities of solution in either direction. It depends upon a caged roller bearing where the individual rollers press against silicon rubber tubing having an internal diameter of 0.5 mm. This assembly was housed in a cylindrical cavity shaped to produce a non-pulsatile flow. The pump is driven by a stepping motor to produce a constant flow rate. It is used to obtain small samples of bathing solution from near the surface of brain slices to monitor efflux of amino acids.