Choosing a polarisation configuration for dynamic light scattering and laser speckle contrast imaging

Laser speckle contrast imaging (LSCI) is applied in various biomedical applications for full-field characterization of blood flow and tissue perfusion. The accuracy of the contrast interpretation and its conversion to the blood flow index depends on specific parameters of the optical system and scattering media. One such parameter is the polarisation of detected light, which is often adjusted to minimize specular reflections and image artefacts. The polarisation's effect on the detected light scattering dynamics and, therefore, the accuracy of LSCI data interpretation requires more detailed investigation. In this study, we used LSCI and Dynamic Light Scattering Imaging to evaluate the effects of the detected light polarisation when imaging perfusion in the mouse cortex. We found that cross-polarisation results in a shorter decorrelation time constant, a higher coherence degree and stronger dynamic scattering compared to the parallel-polarisation or no-polariser configurations. These results support the cross-polarisation configuration as the most optimal for brain cortex imaging and suggest against direct or calibrated comparisons between the contrast recordings made with different polarisation configurations.