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Updated: Jul 2, 2026
14:09
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
15.6K
Complex optically computed phase microscopy for label-free sub-cellular imaging
Xuan Liu1, Yuwei Liu1, Shupei Yu2
1Department of Electrical and Computer Engineering, New Jersey Institute of Technology, University Heights, Newark, NJ, 07102, USA.
Optics Continuum
|January 15, 2024
Summary
Complex optically computed phase microscopy (complex-OCPM) offers depth-resolved phase imaging. This advanced technique achieves sub-cellular resolution for label-free live cell analysis.
Area of Science:
- Biophotonics
- Optical Imaging
- Microscopy
Background:
- Quantitative phase imaging provides label-free contrast for biological samples.
- Existing methods may face limitations in spatial resolution or depth penetration.
Purpose of the Study:
- To investigate the performance of complex optically computed phase microscopy (complex-OCPM).
- To demonstrate depth-resolved quantitative phase measurement with high spatial resolution.
Main Methods:
- Utilized a low coherence interferometer combined with an innovative optical computation approach.
- Directly measured the complex amplitude of the optical field from the sample.
- Extracted phase information as the argument of the complex signal.
Main Results:
- Achieved depth-resolved quantitative phase measurement.
- Demonstrated high spatial resolution in phase imaging.
- Validated performance using resolution targets and live cells, showing sub-cellular resolution.
Conclusions:
- Complex-OCPM enables high-resolution, label-free imaging of biological samples.
- The technology provides quantitative phase information with depth resolution.
- Sub-cellular resolution was achieved on live cells without exogenous labels.
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