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Updated: Jul 2, 2026
Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Complex optically computed phase microscopy for label-free sub-cellular imaging
Xuan Liu1, Yuwei Liu1, Shupei Yu2
1Department of Electrical and Computer Engineering, New Jersey Institute of Technology, University Heights, Newark, NJ, 07102, USA.
Abstract:
In this study, we investigated a complex optically computed phase microscopy (complex-OCPM) technology. Based on a low coherence interferometer and an innovative optical computation approach, the complex-OCPM imaging system achieves depth resolved quantitative phase measurement. Particularly, the complex-OCPM imaging system directly measures the complex amplitude of the optical field emerging from the sample, extracts the phase as the argument of a complex signal, and achieves a high spatial resolution in phase imaging. We evaluated the performance of complex-OCPM imaging using resolution targets and live cells. Our results show that the complex-OCPM system achieves quantitative phase imaging with sub-cellular resolution on label-free cells.
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