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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
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Complex optically computed phase microscopy for label-free sub-cellular imaging.

Xuan Liu1, Yuwei Liu1, Shupei Yu2

  • 1Department of Electrical and Computer Engineering, New Jersey Institute of Technology, University Heights, Newark, NJ, 07102, USA.

Optics Continuum
|January 15, 2024
PubMed
Summary
This summary is machine-generated.

Complex optically computed phase microscopy (complex-OCPM) offers depth-resolved phase imaging. This advanced technique achieves sub-cellular resolution for label-free live cell analysis.

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Area of Science:

  • Biophotonics
  • Optical Imaging
  • Microscopy

Background:

  • Quantitative phase imaging provides label-free contrast for biological samples.
  • Existing methods may face limitations in spatial resolution or depth penetration.

Purpose of the Study:

  • To investigate the performance of complex optically computed phase microscopy (complex-OCPM).
  • To demonstrate depth-resolved quantitative phase measurement with high spatial resolution.

Main Methods:

  • Utilized a low coherence interferometer combined with an innovative optical computation approach.
  • Directly measured the complex amplitude of the optical field from the sample.
  • Extracted phase information as the argument of the complex signal.

Main Results:

  • Achieved depth-resolved quantitative phase measurement.
  • Demonstrated high spatial resolution in phase imaging.
  • Validated performance using resolution targets and live cells, showing sub-cellular resolution.

Conclusions:

  • Complex-OCPM enables high-resolution, label-free imaging of biological samples.
  • The technology provides quantitative phase information with depth resolution.
  • Sub-cellular resolution was achieved on live cells without exogenous labels.