Gastrodin improves osteoblast function and adhesion to titanium surface in a high glucose environment

Area of Science:

• Biomaterials Science
• Cell Biology
• Pharmacology

Background:

• High glucose environments impair osteoblast biological behavior and osseointegration.
• Oxidative stress is a key mechanism underlying high glucose-induced cellular damage.

Purpose of the Study:

• To investigate gastrodin's effects on osteoblast function and osseointegration on titanium surfaces in a high glucose environment.
• To explore the regulatory mechanisms of gastrodin in this context.

Main Methods:

• Established a high glucose-induced oxidative damage model using MC3T3-E1 cells *in vitro*.
• Assessed gastrodin's impact on oxidative stress, cell viability, osteogenic differentiation, mineralization, migration, and adhesion to titanium.

Main Results:

• Gastrodin reversed high glucose-induced decreases in cell viability, osteogenesis, migration, and adhesion.
• Gastrodin upregulated antioxidant enzymes (Nrf2, HO-1) and osteogenic proteins (RUNX2, BMP2), reducing reactive oxygen species (ROS).
• Gastrodin improved osteoblast cytoskeleton morphology and titanium surface adhesion.

Conclusions:

• Gastrodin protects osteogenesis and osseointegration by upregulating antioxidant enzymes and attenuating oxidative stress.
• Gastrodin shows potential as a therapeutic agent for improving implant osseointegration in diabetic patients.