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Microbial Biosensors01:17

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Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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A light-sensitive protein-based wearable pH biometer.

Wenxue Li1,2, Siyuan Chen3, Shiwang Xie2

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This study introduces a wearable biosensor using bacteriorhodopsin (bR) to detect wound infections by monitoring pH levels. The bR-based device shows a reliable linear relationship between photovoltage ratio and pH, enabling accurate infection monitoring.

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Area of Science:

  • Biophysics
  • Biomedical Engineering
  • Biosensor Technology

Background:

  • Bacteriorhodopsin (bR) is a photosensitive protein that converts light into electrical signals, making it suitable for biosensor applications.
  • Monitoring pH changes is crucial for assessing wound infection status.
  • Existing methods for wound pH monitoring can be invasive or lack real-time capabilities.

Purpose of the Study:

  • To develop and validate a wearable pH biometer based on bacteriorhodopsin for monitoring wound infections.
  • To investigate the pH-sensitive photoelectric properties of bR electrodes.
  • To establish the correlation between photovoltage signals and pH variations in the context of wound healing.

Main Methods:

  • Fabrication of a wearable biometer utilizing bacteriorhodopsin electrodes.
  • Characterization of the photovoltage generation and pH-dependent response of the bR electrode.
  • Establishing the linear relationship between the ratio of negative to positive photovoltage (Vn/Vp) and pH.
  • In vitro validation using a rat wound infection model to assess real-time pH monitoring.

Main Results:

  • The bR electrode exhibits a pH-sensitive photoelectric effect, generating distinct photovoltages under light and darkness.
  • A strong linear correlation (R² = 0.9911) was observed between the Vn/Vp ratio and pH in the range of 4.0-10.0.
  • In vitro experiments demonstrated the biometer's capability to accurately track pH fluctuations associated with wound infection in a rat model.

Conclusions:

  • A novel wearable bacteriorhodopsin-based pH biometer has been successfully developed for wound infection monitoring.
  • The device leverages the pH-dependent photoelectric properties of bR for sensitive and reliable pH detection.
  • This technology offers a promising non-invasive tool for real-time assessment of wound status and infection progression.