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Related Concept Videos

Channel Rhodopsins01:11

Channel Rhodopsins

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Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
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Construction and Setup of a Bench-scale Algal Photosynthetic Bioreactor with Temperature, Light, and pH Monitoring for Kinetic Growth Tests
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Bacteriorhodopsin-Based pH Sensor for Cell Culture Condition Regulation.

Jiayin Huang1,2,3,4, Shiwang Xie2,5, Haoqi Fan2,6

  • 1Key Laboratory of Infectious Immune and Antibody Engineering of Guizhou Province, Engineering Research Center of Cellular Immunotherapy of Guizhou Province, School of Biology and Engineering, Guizhou Medical University, Guiyang 561113, China.

Materials (Basel, Switzerland)
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Summary

This study introduces a novel pH biosensor using bacteriorhodopsin (bR), a light-sensitive protein. This non-invasive sensor accurately monitors pH in cell cultures, improving experimental reliability.

Keywords:
bacteriorhodopsincell culturepH sensor

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Area of Science:

  • Biotechnology
  • Biomedical Engineering
  • Biophysics

Background:

  • Precise pH monitoring is vital for cell culture and biotechnology.
  • Traditional pH sensors face limitations like fragility and cytotoxicity.
  • Bacteriorhodopsin (bR) offers a potential alternative due to its light-sensitive properties.

Purpose of the Study:

  • To develop and demonstrate a novel, flexible pH biosensor utilizing bacteriorhodopsin.
  • To enable real-time, non-invasive pH monitoring in physiological ranges.
  • To validate the sensor's utility in cell culture applications.

Main Methods:

  • Fabrication of an integrated bR-based electrochemical electrode.
  • Measurement of photoelectric signals generated by bR in response to pH changes (6.0-8.5).
  • Integration of the sensor into cell well plates for in-situ monitoring.

Main Results:

  • The bR-based sensor demonstrated a strong correlation (R² = 0.977) between photo-generated current and pH.
  • The sensor operates effectively within the physiological pH range.
  • Successful validation in tracking pH fluctuations during cell growth.

Conclusions:

  • The novel bR-based biosensor provides a robust, non-invasive method for real-time pH monitoring in cell cultures.
  • This technology enhances cell culture research by offering valuable insights into metabolic processes.
  • Future work will focus on improving sensor sensitivity, stability, and multi-parameter integration.