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Sample Injection for Real-Time Analysis (SIRTA) Using GC-MS with Cold EI.

Oneg Elkabets1, Benny Neumark1, Aviv Amirav1,2

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Journal of the American Society for Mass Spectrometry
|January 18, 2024
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Summary

A new Sample Injection for Real-Time Analysis (SIRTA) method uses Gas Chromatography-Mass Spectrometry with Cold Electron Ionization (GC-MS with Cold EI) for rapid analysis. This cost-effective technique achieves real-time analysis without GC separation, analyzing samples in under a minute.

Keywords:
GC-MS with Cold EISIRTAflow injectionreal-time analysis

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Area of Science:

  • Analytical Chemistry
  • Mass Spectrometry
  • Chromatography

Background:

  • Real-time analysis (RTA) demands advanced methods beyond current ambient desorption ionization techniques.
  • Liquid sample injection offers an alternative for RTA without modifying existing LC-MS instruments.

Purpose of the Study:

  • Introduce a novel RTA approach named Sample Injection for Real-Time Analysis (SIRTA).
  • Utilize Gas Chromatography-Mass Spectrometry with Cold Electron Ionization (GC-MS with Cold EI) for cost-effective RTA.

Main Methods:

  • Replaced standard GC column with a short capillary (1 m long, 0.1 mm I.D.) connecting GC injector to Cold EI MS transfer-line.
  • Dissolved samples (∼1 mL) in methanol or other solvents and injected via autosampler using a standard syringe, bypassing GC separation.
  • Employed Cold EI's open ion source to manage solvent elution and enhance molecular ions.

Main Results:

  • Achieved rapid analysis times of 0.2-0.7 minutes per sample, enabling consecutive analyses.
  • Demonstrated SIRTA Cold EI analysis of 12 compounds and 7 mixtures, including drugs, cannabis, petroleum, and synthetic organics up to 800 g/mol.
  • Confirmed zero added cost and no need for additional instrumentation.

Conclusions:

  • SIRTA with Cold EI is a viable, cost-effective method for real-time analysis of diverse samples.
  • The technique offers rapid, consecutive analyses with enhanced molecular ion detection.
  • Applicable to a wide range of organic compounds and complex mixtures.