Synthesized uniform-different sizes silver nanoparticles using TSC and SBH simultaneously for antibacterial...

Area of Science:

• Nanotechnology
• Materials Science
• Microbiology

Background:

• Silver nanoparticles (AgNPs) exhibit potent antimicrobial properties.
• Controlling AgNP size and synthesis is crucial for optimizing antibacterial efficacy.
• Investigating AgNP interactions with resistant bacterial strains is essential for developing new treatments.

Purpose of the Study:

• To synthesize uniform silver nanoparticles (AgNPs) using a novel dual-reducing agent method.
• To evaluate the impact of AgNP size and concentration on antibacterial activity.
• To compare the efficacy of AgNPs against *Pseudomonas aeruginosa* (PA) and *Staphylococcus aureus* (SA).

Main Methods:

• Controlled synthesis of AgNPs using sodium borohydride (SBH) and trisodium citrate (TSC).
• Characterization of AgNPs using Transmission Electron Microscopy (TEM) and Dynamic Light Scattering (DLS).
• Antimicrobial activity assessed via the disc diffusion method against PA and SA.

Main Results:

• Antibacterial activity of AgNPs is dependent on concentration and particle size.
• Optimal antibacterial effect observed at 50 μg/ml concentration.
• Smaller AgNPs demonstrated significantly higher antibacterial efficacy.
• The dual-reducing agent method yielded uniform AgNPs, enhancing antibacterial performance.

Conclusions:

• The simultaneous use of TSC and SBH enables controlled synthesis of uniform AgNPs.
• Smaller AgNPs exhibit enhanced antibacterial properties against *P. aeruginosa* and *S. aureus*.
• Optimized AgNP concentration and size present a promising strategy for combating resistant bacterial infections.