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Updated: Jul 5, 2025

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response
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Budding yeast as an ideal model for elucidating the role of N6-methyladenosine in regulating gene expression

Waleed S Albihlal1, Wei Yee Chan1, Folkert J van Werven1

  • 1The Francis Crick Institute, Cell Fate and Gene Regulation Laboratory, London, UK.

Yeast (Chichester, England)
|January 19, 2024
PubMed

Insights

N6-methyladenosine (m6A), a key mRNA modification, is regulated during yeast meiosis. This modification impacts mRNA translation and decay, offering insights into gene expression regulation.

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Yeast Genetics

Background:

  • N6-methyladenosine (m6A) is a prevalent mRNA modification crucial for gene regulation.
  • The m6A methyltransferase complex (MTC) installs m6A marks, recognized by YTH domain proteins.
  • m6A plays roles in mRNA fate determination, including translation and decay.

Purpose of the Study:

  • To review recent advances in understanding m6A regulation and roles during yeast meiosis.
  • To explore the functions of m6A in mRNA translation and decay in yeast.
  • To highlight yeast as a model for studying m6A-mediated posttranscriptional gene regulation.

Main Methods:

  • Review of existing literature on m6A modification in yeast meiosis.
  • Analysis of m6A's impact on mRNA translation and decay pathways.
  • Comparative study of m6A regulation across eukaryotic systems.

Main Results:

  • m6A is installed on thousands of mRNAs during yeast meiosis by a conserved MTC.
  • The YTH domain protein Mrb1/Pho92 recognizes m6A-modified mRNAs in yeast.
  • m6A modification influences mRNA translation efficiency and decay rates.

Conclusions:

  • Yeast meiosis provides a powerful model for dissecting fundamental m6A regulatory mechanisms.
  • Understanding m6A in yeast expands knowledge of mRNA modifications and posttranscriptional regulation.
  • Insights from yeast research contribute to the broader understanding of eukaryotic gene expression.
Keywords:
Ime4N6‐methyladenosinePho92RNA stabilitymeiosismethyltransferase complex

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