Covalent-organic framework nanobionics for robust cytoprotection

Area of Science:

• Materials Science
• Biotechnology
• Nanotechnology

Background:

• Covalent-organic frameworks (COFs) offer tunable properties for advanced applications.
• Protecting living cells from environmental stressors is crucial for biotechnology.
• Existing nanomaterials often lack the robustness needed for extreme conditions.

Purpose of the Study:

• To develop and characterize a novel, in situ formed COF nanocoating on living cells.
• To evaluate the nanocoating's protective capabilities against various lethal stressors.
• To explore the potential of enzyme-functionalized COF nanocoatings for enhanced microbial function.

Main Methods:

• In situ synthesis of COF nanocoating on yeast cells.
• Exposure of coated cells to stressors: high temperature, extreme pH, UV radiation, metal ions, organic pollutants, oxidative stress.
• Assessment of cell viability and metabolic activity (fermentation, ethanol production).

Main Results:

• The COF nanocoating demonstrated exceptional durability and resistance to a wide array of lethal stressors.
• Significant enhancement in cell survival was observed under high temperature and acidic conditions.
• Enzyme-functionalized COF nanocoatings enabled yeast fermentation and ethanol production under strong oxidative stress.

Conclusions:

• Durable COF nanocoatings provide a robust platform for protecting living cells.
• This technology enables microbial utilization in previously inaccessible harsh environments.
• COF nanocoatings represent a promising advancement in cell protection and bioengineering.