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Updated: Jul 5, 2025

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
Published on: June 21, 2011
Comparison of large-volume 3D reconstruction using plasma FIB-SEM and X-ray CT
Ryuji Yoshida1, Yasutoshi Mizuta2, Takeharu Kato1
1Nanostructures Research Laboratory, Japan Fine Ceramics Center, 2-4-1, Mutsuno, Atsuta-ku, Nagoya 456-8587, Japan.
Abstract:
We demonstrated large-volume three-dimensional (3D) reconstruction using plasma-focused ion beam-scanning electron microscopy (PFIB-SEM). We successfully reconstructed a 750 μm (W) × 143 μm (H) × 310 μm (D) volume at a resolution of 200 nm/pix from 1550 SEM backscattered electron images of a Li-ion battery cathode sheet. The PFIB-SEM system was found to be capable of acquiring and reconstructing larger volume 3D datasets than X-ray computed tomography, and with higher resolution and contrast.
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