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Related Experiment Video

Updated: Jul 5, 2025

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture
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A Modular Microfluidic Organoid Platform Using LEGO-Like Bricks.

Daniel J Carvalho1, Anna M Kip2, Andreas Tegel3

  • 1Department of Instructive Biomaterials Engineering, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, 6229 ER, The Netherlands.

Advanced Healthcare Materials
|January 22, 2024
PubMed
Summary

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This summary is machine-generated.

A new modular fluidic circuit board (FCB) system integrates organoids with organ-on-a-chip (OoC) technology. This flexible platform simplifies dynamic culture of 3D cell models, overcoming current limitations.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Tissue Engineering

Background:

  • Current 3D in vitro models, including organoids, face limitations in standard organ-on-a-chip (OoC) systems.
  • Integrating organoids into OoCs presents technical challenges, leading to complex and inefficient cell culture protocols.
  • There is a need for adaptable microfluidic platforms to incorporate complex 3D in vitro models.

Purpose of the Study:

  • To develop a flexible and user-friendly microfluidic platform for the dynamic culture of organoids.
  • To overcome the limitations of current methods for integrating organoids into OoC systems.
  • To facilitate the combination of organoid technology with advanced microfluidic platforms.

Main Methods:

  • Development of a modular, tubeless fluidic circuit board (FCB) system.
Keywords:
LEGO®microfluidicsmodularorganoidsorgan‐on‐a‐chipthyroid

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  • Fabrication of the FCB by milling channels in polycarbonate plates and thermal bonding.
  • Integration of LEGO-like fluidic interconnectors and lock-and-play clamps for cell culture bricks.
  • Dynamic culture of embryonic stem cell-derived thyroid follicles using the developed platform.

    • Main Results:

    • The developed FCB system allows for easy and rapid (un)loading of organoids.
    • Thyroid organoids were successfully cultured on-chip for up to 10 days without structural or functional loss.
    • A multiplexed perfusion experiment demonstrated the system's capability for parallel experiments.

    Conclusions:

    • The modular FCB platform offers a flexible and efficient solution for integrating organoids with microfluidic systems.
    • This technology simplifies dynamic culture protocols for 3D in vitro models.
    • The system shows promise for advancing organoid and OoC research by enabling synergistic combination.