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Updated: Jul 5, 2025

Partial Sciatic Nerve Ligation: A Mouse Model of Chronic Neuropathic Pain to Study the Antinociceptive Effect of Novel Therapies
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LncRNA 51325 Alleviates Bone Cancer Induced Hyperalgesia Through Inhibition of Pum2

Yahui Wang1,2, Chengfei Xu3, Peng Liu2

  • 1Department of Anesthesiology and Pain Research Center, the Affiliated Hospital of Jiaxing University, Jiaxing, 314001, People's Republic of China.

Journal of Pain Research
|January 22, 2024
PubMed
Abstract

Insights

Bone cancer pain (BCP) is challenging. Spinal lncRNA51325 inhibits Pum2, reducing pain and anxiety, offering new therapeutic targets for BCP.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Oncology

Background:

  • Bone cancer pain (BCP) is a significant challenge in cancer metastasis.
  • Long non-coding RNAs (lncRNAs) show potential for neuropathic pain management.
  • The role of lncRNAs in nociception processing is not well understood.

Purpose of the Study:

  • To investigate the role of spinal lncRNA ENSRNOG00000051325 (lncRNA51325) in bone cancer pain.
  • To explore the relationship between lncRNA51325 and Pumilio RNA binding family member 2 (Pum2).
  • To elucidate the therapeutic potential of lncRNA51325 in BCP.

Main Methods:

  • Established a rat model of BCP by injecting Walker 256 cells into the tibial plateau.
  • Assessed pain behaviors and anxiety-like responses using von-Frey, gait analysis, and open field tests.
  • Analyzed the expression of lncRNA51325 and Pum2 in spinal cord tissue.

Main Results:

  • Overexpression of lncRNA51325 attenuated mechanical allodynia in BCP rats.
  • Knockdown of lncRNA51325 induced pain and anxiety in naïve rats.
  • lncRNA51325 negatively modulated Pum2 expression, suppressing its levels in BCP rats.

Conclusions:

  • lncRNA51325 plays a crucial role in regulating bone cancer pain by inhibiting Pum2.
  • Targeting lncRNA51325 offers a promising therapeutic strategy for nociceptive hypersensitivity in BCP.
  • This study provides insights into the molecular mechanisms underlying BCP.
Keywords:
Pum2bone cancer painlong non-coding RNAspinal cord

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