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Sequencing Reveals miRNAs Enriched in the Developing Mouse Enteric Nervous System.

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  • 1The Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA.

Non-Coding RNA
|January 22, 2024
PubMed
Summary

Researchers identified key microRNAs (miRNAs) in the developing enteric nervous system (ENS). However, they found that miR-137 loss did not impact ENS development or survival in mice, challenging previous findings.

Keywords:
Hirschsprung diseaseenteric nervous systemmiR-124miR-137miR-27bmiR-488miR-9smRNA-seq

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Area of Science:

  • Developmental biology
  • Neuroscience
  • Genetics

Background:

  • The enteric nervous system (ENS) is crucial for gut function.
  • Hirschsprung disease (HSCR) arises from ENS developmental defects.
  • MicroRNA (miRNA) alterations are linked to HSCR, but the normal developing ENS miRNA profile is unclear.

Purpose of the Study:

  • To characterize the miRNA landscape in the developing ENS.
  • To investigate the role of specific miRNAs, including miR-137, in ENS development.

Main Methods:

  • Small RNA sequencing (smRNA-seq) and mRNA sequencing (mRNA-seq) were performed on mouse ENS precursor cells.
  • RNA was compared between ENS precursor cells and other developing bowel cells.
  • Genetic manipulation was used to assess the function of miR-137 in vivo.

Main Results:

  • 73 miRNAs were significantly enriched in the developing ENS.
  • Top 5 conserved miRNAs include miR-9, miR-27b, miR-124, miR-137, and miR-488.
  • Loss of miR-137 in various neural crest lineages did not affect mouse survival or ENS development.

Conclusions:

  • This study provides a comprehensive miRNA profile of the developing ENS.
  • The findings challenge the previously reported role of miR-137 in ENS development.
  • Further research is needed to understand ENS development and HSCR pathogenesis.