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Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model
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Novel method for determining when a field-collected donor unit is sufficiently full.

Mark H Yazer1, Kendall Werneiwski2, Patrick Thompson3

  • 1Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.

Transfusion
|January 22, 2024
PubMed
Summary

A novel string method accurately determines when whole blood (WB) units are full for field collections. Precut strings can be marked on bags, ensuring optimal fill levels for immediate transfusion needs.

Keywords:
buddy transfusioncollectionfieldfreshmeasurementspecial forceswarmwhole blood

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Area of Science:

  • Blood banking and transfusion medicine
  • Field medical operations
  • Biomedical engineering

Background:

  • Whole blood (WB) collections are vital for immediate, downrange administration.
  • Accurate determination of collection unit fullness is critical for field operations.
  • This study introduces and evaluates a new method for assessing WB collection completeness.

Purpose of the Study:

  • To test a novel, simple method for determining when a whole blood collection unit is sufficiently full.
  • To provide a practical solution for field blood collection challenges.
  • To establish a reliable indicator for optimal blood unit collection in diverse settings.

Main Methods:

  • Measured the empty space in WB units using a string method, noting where the blood column intersected the string.
  • Held WB units off the ground and placed string at the donor tubing entry point.
  • Weighed WB units to correlate with volume measurements.

Main Results:

  • Evaluated 15 WB units from various hospitals/blood centers.
  • The commonly used Terumo Imuflex SP bag showed a median string length of 9 mm (range: 2-24 mm).
  • Median weight for these bags was 565.1 g (range: 524.8-636.7 g).

Conclusions:

  • Precut strings can be prepared to specific lengths based on bag type for field collections.
  • Marking bags with string measurements provides a clear indicator of when a unit is full.
  • This method offers a practical and effective approach to managing field WB collections.