Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interactions and Conformational Plasticity of Human Uncoupling Protein 1 in Response to Small-Molecule Analogues: Insights from Molecular Dynamics Simulations.

ACS omega·2026
Same author

Enhancing propagation and purification efficiency of M13 bacteriophage for improved phage display applications.

Biology methods & protocols·2026
Same author

Correction to "Phase Characterization and Bioactivity Evaluation of Nucleic Acid-Encapsulated Biomimetically Mineralized ZIF-8".

ACS applied materials & interfaces·2026
Same author

Clinical observations of tetanus toxin plus decoy, Snoretox-1, a novel targeted neuromuscular stimulant, in a pilot study of 6 British bulldogs with BOAS.

Veterinary journal (London, England : 1997)·2026
Same author

Distinct Bioactive Surface Coating Modulates Chronic Toxicity and Recovery Pathways in Silver-Nanoparticle-Exposed Cells.

ACS applied bio materials·2026
Same author

Systematic evaluation of CrRNA design parameters for optimized Cas13d-mediated RNA targeting in chicken cells.

Functional & integrative genomics·2025

Related Experiment Video

Updated: Jul 5, 2025

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

23.1K

Solid Lipid Nanoparticles Delivering a DNA Vaccine Encoding Helicobacter pylori Urease A Subunit: Immune Analyses

Jasmine E Francis1, Ivana Skakic1, Debolina Majumdar1

  • 1School of Science, RMIT University, 264 Plenty Road, Bundoora, VIC 3083, Australia.

International Journal of Molecular Sciences
|January 23, 2024
PubMed
Summary
This summary is machine-generated.

Novel nanoparticles carrying a DNA vaccine for Helicobacter pylori were developed. These lipoplex-A nanoparticles successfully induced antigen-specific antibodies and T cell responses in mice.

Keywords:
DNA vaccineHelicobacter pylorisolid lipid nanoparticlesurease alpha subunit

More Related Videos

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation
06:38

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

Published on: May 3, 2018

8.5K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.6K

Related Experiment Videos

Last Updated: Jul 5, 2025

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

23.1K
Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation
06:38

Novel Method of Plasmid DNA Delivery to Mouse Bladder Urothelium by Electroporation

Published on: May 3, 2018

8.5K
A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain
08:07

A "Plug-And-Display" Nanoparticle Vaccine Platform Based on Outer Membrane Vesicles Displaying SARS-CoV-2 Receptor-Binding Domain

Published on: July 25, 2022

2.6K

Area of Science:

  • Nanotechnology
  • Vaccinology
  • Immunology

Background:

  • Helicobacter pylori infection is a significant global health concern.
  • Effective vaccines are needed to combat H. pylori.
  • DNA vaccines offer a promising platform for vaccine development.

Purpose of the Study:

  • To develop and evaluate novel solid lipid nanoparticles (SLN-A) for DNA vaccine delivery.
  • To assess the immunogenicity of lipoplex-A nanoparticles in a mouse model of H. pylori infection.
  • To investigate the potential for tailoring immune responses using this delivery system.

Main Methods:

  • Synthesis of solid lipid nanoparticles (SLN-A) containing monophosphoryl lipid A.
  • Complexation of SLN-A with a DNA vaccine encoding the urease alpha subunit of H. pylori to form lipoplex-A.
  • Immunization of mice with lipoplex-A nanoparticles.
  • Analysis of antigen-specific antibody levels (IgG1, IgG2c) and gastric CD4+ T cell influx.

Main Results:

  • Lipoplex-A nanoparticles efficiently formed complexes with the DNA vaccine.
  • Vaccination induced high levels of antigen-specific antibodies and gastric CD4+ T cells.
  • Different vaccination regimens (prime-boost vs. two doses) elicited distinct antibody profiles (IgG1 vs. IgG2c).

Conclusions:

  • Lipoplex-A nanoparticles are a viable system for delivering DNA vaccine antigens.
  • This system can induce significant antigen-specific immune responses.
  • The ability to tailor immune responses suggests potential for optimized vaccine strategies against H. pylori.