Using a 3D Silicon Micro-Channel Device and Raman Spectroscopy for the Analysis of Whole Blood and Abnormal Blood

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Spectroscopy

Background:

• Blood testing is vital for disease diagnosis, biomarker discovery, and personalized medicine.
• Accurate and rapid data collection is essential for clinical applications.
• Raman spectroscopy offers a non-destructive method for analyzing biological samples.

Purpose of the Study:

• To utilize Raman spectroscopy for comprehensive biological information extraction from blood samples.
• To analyze whole blood, plasma, and serum for distinct biological characteristics.
• To explore the potential of a novel 3D silicon micro-channel device for medical blood testing.

Main Methods:

• Short-wavelength (532 nm green light) Raman scattering spectroscopy was employed.
• Analysis was performed on whole blood, plasma, and serum samples.
• Characteristic Raman peaks for hemoglobin and beta-carotene were identified.

Main Results:

• Whole blood analysis revealed high hemoglobin content with characteristic Raman peaks.
• Plasma and serum analysis detected beta-carotene, identified by specific Raman peaks.
• The study demonstrated the capability of Raman spectroscopy to differentiate blood components.

Conclusions:

• Raman spectroscopy effectively extracts detailed biological information from blood samples.
• The developed 3D silicon micro-channel device shows promise for advanced medical blood testing.
• This technology could enable real-time disease detection and personalized health monitoring.