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Updated: Jul 5, 2025

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
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Oncolytic Myxoma virus Increases Autophagy in Multiple Myeloma

Alpay Yeşilaltay1, Dilek Muz2, Berna Erdal3

  • 1Başkent University İstanbul Hospital, Department of Hematology, İstanbul, Türkiye

Turkish Journal of Haematology : Official Journal of Turkish Society of Haematology
|January 23, 2024
PubMed
Abstract

Insights

Myxoma virus (MYXV) increases autophagy in multiple myeloma cells, demonstrating an anti-myeloma effect. This study explored MYXV

Area of Science:

  • Oncology
  • Virology
  • Cell Biology

Background:

  • Multiple myeloma is a prevalent hematological malignancy affecting plasma cells.
  • Current treatments for multiple myeloma have limitations in improving patient survival rates.
  • Oncolytic viruses are being explored as a therapeutic strategy for cancer treatment.

Purpose of the Study:

  • To investigate the impact of Myxoma virus (MYXV) on autophagy in multiple myeloma cells.
  • To evaluate MYXV as a potential therapeutic agent against multiple myeloma.

Main Methods:

  • Analyzed autophagy markers (ATG-5, p62, Beclin-1, LC3B) and apoptosis marker (Bcl-2) protein expression.
  • Utilized human U-266 and mouse MOPC-315 myeloma cell lines.
  • Employed transmission electron microscopy (TEM) to visualize autophagosomes.

Main Results:

  • MYXV treatment increased ATG-5 and Beclin-1 expression within 24 hours in myeloma cell lines.
  • A significant increase in the late autophagy indicator LC3B was observed at 48 hours post-MYXV treatment.
  • TEM confirmed the presence of autophagosomes in MYXV-treated myeloma cells.

Conclusions:

  • Myxoma virus (MYXV) induces autophagy in multiple myeloma cells.
  • MYXV exhibits an anti-myeloma effect through the induction of autophagy.
Keywords:
AutophagosomeMyxoma virusMultiple myelomaAutophagy

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