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Rosa roxburghii Tratt Pomace Crude Extract Inactivates Cronobacter sakazakii Isolated from Powdered Infant Formula
Peng Fei1, Jing Xu1, Jinlan Xie1
1School of Zhang Zhongjing Health Care and Food, Nanyang Institute of Technology, Nanyang, China.
Foodborne Pathogens and Disease
|January 24, 2024
Summary
Rosa roxburghii pomace extract effectively inactivates Cronobacter sakazakii, a foodborne pathogen in infant formula. This natural extract disrupts bacterial cells and shows potential as a disinfectant for packaging materials.
Area of Science:
- Food Microbiology
- Natural Product Chemistry
- Food Safety
Background:
- Cronobacter sakazakii is a significant foodborne pathogen commonly found in powder infant formula (PIF).
- Effective inactivation strategies are crucial to prevent contamination and ensure infant safety.
Purpose of the Study:
- To evaluate the antimicrobial efficacy of Rosa roxburghii Tratt pomace crude extract (RRPCE) against Cronobacter sakazakii.
- To elucidate the mechanism of action of RRPCE in inactivating the pathogen.
- To assess RRPCE's potential as a natural disinfectant for PIF packaging surfaces.
Main Methods:
- Determined Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of RRPCE.
- Investigated RRPCE's effects on bacterial intracellular adenosine 5'-triphosphate (ATP), reactive oxygen species (ROS), membrane potential, and nucleic acid/protein leakage.
- Assessed RRPCE's impact on Cronobacter sakazakii cell morphology and biofilm inactivation on various packaging materials.
Main Results:
- RRPCE exhibited MIC and MBC values of 7.5 and 15 mg/mL, respectively, against Cronobacter sakazakii.
- RRPCE treatment led to significant intracellular ATP reduction and ROS increase, indicating cell damage.
- Significant cell membrane depolarization, leakage of cellular components, and morphological damage were observed.
Conclusions:
- RRPCE effectively inactivates Cronobacter sakazakii by disrupting cellular integrity and function.
- RRPCE demonstrates potent bactericidal activity against biofilms on diverse packaging surfaces.
- RRPCE holds promise as a natural disinfectant for powder infant formula packaging and containers.
