Performance and biocompatibility of OSTEMER 322 in cell-based microfluidic applications

Area of Science:

• Biomaterials Science
• Microfluidics
• Cell Biology

Background:

• Off-Stoichiometry Thiol-ene and Epoxy (OSTE+) polymers are increasingly used in microfluidics.
• The biocompatibility of OSTEMER 322, a specific formulation, requires thorough investigation for cell-based applications.

Purpose of the Study:

• To evaluate the biocompatibility of OSTEMER 322 and its surface modifications.
• To assess the impact of OSTEMER 322 on various cell types for microfluidic applications.

Main Methods:

• Cell viability and confluence assays were performed on multiple cell lines.
• Surface characterization included contact angle, zeta potential, and X-ray photoelectron spectroscopy.
• Mass spectrometry was used to detect leaching constituents.

Main Results:

• OSTEMER 322 demonstrated sufficient cell viability for standard cell culture.
• Surface modifications did not negatively impact cell viability.
• Mass spectrometry confirmed no leaching from the OSTEMER material.

Conclusions:

• OSTEMER 322 is a suitable material for cell-based assays in microfluidic devices.
• The material exhibits adequate biocompatibility for fibroblasts and epithelial cells.
• OSTE+ technology offers a promising platform for lab-on-a-chip applications.