Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The mitochondrial protease, LonP1, is a potential cardioprotective target for attenuating doxorubicin-induced cardiomyocyte death.

Journal of translational medicine·2026

Same author

The Integrated Stress Response Protein, eIF2α, Modulates UPR<sup>mt</sup> Signaling and Cell Death in Doxorubicin Treated Human Cardiac Cells.

Cardiovascular toxicology·2026

Same author

Prediction and Characterization of Glycated Peptides and Proteins using Hydrophilic Interaction Liquid Chromatography coupled with Mass Spectrometry (HILIC-MS).

Journal of biomolecular techniques : JBT·2026

Same author

Preview of the 2026 ABRF Annual Meeting.

Journal of biomolecular techniques : JBT·2026

Same author

Why Attending ABRF Conferences are Important to Me.

Journal of biomolecular techniques : JBT·2026

Same author

Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2025 Conference Workshops.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2025

Search research articles

Related Experiment Video

Updated: Jul 5, 2025

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

A Survey on Core Flow Cytometry Facilities: Instrument Maintenance, Usage, and Funding.

Sonal Priya¹, Kathleen Brundage², Ron Orlando¹

¹Department of Chemistry University of Georgia AthensGeorgia30602 USA.

Journal of Biomolecular Techniques : JBT

|January 25, 2024

Summary

Flow cytometry shared resource laboratories (SRLs) rely on various instruments. This survey reveals insights into flow cytometer maintenance, usage, and replacement factors within these core facilities.

More Related Videos

Characterization of Aquatic Biofilms with Flow Cytometry

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

Related Experiment Videos

Last Updated: Jul 5, 2025

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Characterizing Microbiome Dynamics – Flow Cytometry Based Workflows from Pure Cultures to Natural Communities

Published on: July 12, 2018

Characterization of Aquatic Biofilms with Flow Cytometry

Characterization of Aquatic Biofilms with Flow Cytometry

Published on: June 6, 2018

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

Area of Science:

Biotechnology
Life Sciences
Research Infrastructure

Background:

Flow cytometry is crucial for research in immunology, molecular biology, and cancer biology.
Shared Resource Laboratories (SRLs) heavily utilize flow cytometry instruments.
Different flow cytometer types (analyzers, sorters, imaging, mass) present unique maintenance challenges.

Purpose of the Study:

To investigate instrument maintenance and turnover in flow cytometry SRLs.
To understand factors influencing flow cytometer uptime, usage, and replacement.
To gather data on routine maintenance, deep cleaning, quality control, and funding sources.

Main Methods:

An independent online survey was conducted.
Data collected from 146 core facilities.
Questions focused on instrument uptime, usage, maintenance, costs, cleaning, QC, funding, and replacement reasons.

Main Results:

The survey provides an operational overview of typical SRLs.
Responses reflect researchers' practical experiences with flow cytometer management.
Key data on instrument performance and user-reported issues were compiled.

Conclusions:

Understanding maintenance and turnover is vital for SRL efficiency.
Survey results offer valuable insights for managing flow cytometry core facilities.
This study highlights the practical aspects of flow cytometer utilization and upkeep.