Related Experiment Video
Updated: Jul 5, 2025
08:48
Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
Published on: October 13, 2011
13.1K
Remote focusing optical tweezers for 3D imaging
Ting Ting Zheng1, Yuan Tian2, Yong Jiang3
1Heilongjiang Industrial Technology Research Institute, Harbin 150080, China.
The Review of Scientific Instruments
|January 25, 2024
Summary
This study introduces a remote focusing optical tweezer system that enables precise axial manipulation of microscopic samples. The innovative design allows for three-dimensional imaging with sectioning microscopes, useful in radiation biology.
Area of Science:
- Optical physics
- Biophysics
- Microscopy
Background:
- Aberrations in optical systems limit precise manipulation.
- Remote focusing is crucial for applications with sample-side constraints.
Purpose of the Study:
- To develop a remote focusing optical tweezer system.
- To enable precise axial manipulation and 3D imaging.
- To overcome limitations of traditional optical tweezers.
Main Methods:
- Utilized a 4f symmetrical optical system for aberration compensation.
- Employed mirror positioning in the focal region for axial trap adjustment.
- Integrated the optical tweezer with a confocal microscope for 3D imaging.
Main Results:
- Achieved axial trap adjustment beyond 100 micrometers.
- Demonstrated successful translation of microspheres and cells.
- Obtained sectioning images of samples during axial translation.
Conclusions:
- The developed optical tweezer system effectively compensates for aberrations.
- Enables versatile 3D imaging and sample manipulation.
- Offers significant advantages for applications like radiation biology.
