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Updated: Jul 5, 2025

Generating Spheroids from Various Chondrocytes using Low-Adhesive Conditions under Gravity and Homemade Mini-Bioreactors
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Scaffolded Chondrogenic Spheroid-Engrafted Model.

Shuang Liu1

  • 1Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. liussmzk@m.ehime-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|January 25, 2024
PubMed
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Mesenchymal stem cells (MSCs) combined with atelocollagen promote cartilage regeneration by enhancing chondrogenesis and host cell recruitment. This approach enables effective screening of anti-rheumatoid agents and evaluation of cartilage repair in arthritis models.

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Orthopedics

Background:

  • Mesenchymal stem cells (MSCs) are crucial for skeletal regeneration due to their multipotent differentiation capabilities.
  • Cartilage regeneration strategies often leverage MSCs for their potential to form chondrocytes.
  • Atelocollagen is explored as a biomaterial to support MSC-based therapies.

Purpose of the Study:

  • To investigate the use of atelocollagen-scaffolded MSC spheroids for enhanced cartilage regeneration.
  • To evaluate the efficacy of this system for screening anti-rheumatoid agents ex vivo.
  • To assess the potential for in vivo transplantation in an arthritis model for cartilage self-repair evaluation.

Main Methods:

  • Pre-formation of chondrogenic MSC spheroids using allergenic neutralized type I atelocollagen.
Keywords:
Atelocollagen scaffoldCartilage regenerationChondrogenic spheroidMesenchymal stem cellXenograft model

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  • Utilizing these spheroids for large-scale ex vivo screening of anti-rheumatoid agents.
  • In vivo transplantation of atelocollagen-scaffolded chondrogenic spheroids into a humanized xenografted arthritis model.

    • Main Results:

    • Atelocollagen facilitated cellular condensation and chondrogenic differentiation of MSCs.
    • The system successfully recruited host MSCs, which differentiated into chondrocyte-like cells.
    • The approach allowed for qualitative and quantitative evaluation of cartilage self-repair in vivo.

    Conclusions:

    • Atelocollagen-supported MSC spheroids offer a promising strategy for cartilage regeneration.
    • This method provides a robust platform for ex vivo drug screening and in vivo therapeutic evaluation in arthritis.
    • The findings highlight the potential of MSCs and atelocollagen for advancing cartilage repair therapies.