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Related Concept Videos

Hybridoma Technology01:31

Hybridoma Technology

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Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
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Antibody Structure

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Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
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The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
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Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
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Production of Neutralizing Antibody.

Erika Takemasa1, Shuang Liu2

  • 1Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan.

Methods in Molecular Biology (Clifton, N.J.)
|January 25, 2024
PubMed
Summary
This summary is machine-generated.

This chapter introduces the hybridoma technique for producing monoclonal antibodies (mAbs), essential for biologic development and rheumatoid arthritis (RA) research. It details methods for generating specific mAbs using synthetic proteoliposomes for immunization and screening.

Keywords:
Cell fusionHybridomaImmunizationMonoclonal antibodyProteoliposome

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Area of Science:

  • Biotechnology
  • Immunology
  • Biopharmaceutical Development

Background:

  • Monoclonal antibodies (mAbs) are crucial in early-stage biologics development due to their specificity, homogeneity, and scalability.
  • mAbs serve as foundational materials for engineering antibody fragments and immunomodulatory antibodies.
  • Established utility of mAbs in basic rheumatoid arthritis (RA) research.

Purpose of the Study:

  • To introduce the fundamental hybridoma technique for monoclonal antibody (mAb) production.
  • To outline the key steps involved in the hybridoma technique, from immunization to purification.
  • To highlight the application of synthetic proteoliposomes for specific membrane protein targeting.

Main Methods:

  • Detailed explanation of the hybridoma technique.
  • Involves animal immunization, cell fusion, and hybridoma screening.
  • Utilizes synthetic proteoliposomes for enhanced immunization and screening of target membrane proteins.

Main Results:

  • The hybridoma technique is presented as a feasible method for mAb production.
  • Demonstrates the successful application of synthetic proteoliposomes in targeting specific membrane proteins.
  • Establishes a foundational protocol for generating high-affinity mAbs.

Conclusions:

  • The hybridoma technique remains a cornerstone for mAb generation in biopharmaceutical research.
  • Synthetic proteoliposomes offer a valuable tool for achieving specific antibody affinities, particularly for membrane proteins.
  • This chapter provides a comprehensive guide to mAb production via hybridoma technology.