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Related Experiment Video

Updated: Jul 5, 2025

Lentivirus Production

Lentivirus Production

Published on: October 2, 2009

Lentiviral Production Platform.

¹Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. liussmzk@m.ehime-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)

|January 25, 2024

Summary

This summary is machine-generated.

Lentiviral transfection enables gene modification in challenging cells for preclinical research. Protocols for lentiviral particle production and concentration are detailed, with applications in gene therapy and viral studies.

Keywords:

Gene silencing Lentivirus SARS-Cov-2 Short-hairpin RNA Transfection

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Last Updated: Jul 5, 2025

Lentivirus Production

Lentivirus Production

Published on: October 2, 2009

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells

Published on: June 19, 2013

Area of Science:

Biotechnology
Gene Therapy
Virology

Background:

Lentiviral vectors are effective for gene modification in various cell types, including immune, non-dividing, and terminally differentiated cells.
Their application extends to preclinical studies, offering advantages over other viral vectors for gene delivery.

Purpose of the Study:

To provide protocols for lentiviral particle production and concentration for gene modification applications.
To highlight the utility of lentiviral systems in preclinical gene therapy and viral research.

Main Methods:

Detailed protocols for the production and concentration of lentiviral particles.
Demonstration of lentiviral vector utility in modifying immune cells, stem cells, neurons, and tumor cells.
Adaptation of the lentiviral platform for producing pseudotyped SARS-CoV-2 for research.

Main Results:

Established methods for generating concentrated lentiviral particles.
Successful gene modification in diverse and challenging cell types.
Demonstrated potential for producing pseudotyped coronaviruses for viral studies.

Conclusions:

Lentiviral transfection is a versatile tool for gene modification in preclinical research and gene therapy.
The described protocols facilitate lentiviral particle production and concentration.
The lentiviral platform offers alternative applications in virology research, including SARS-CoV-2 studies.