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Related Concept Videos

Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

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Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
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Stem Cell Therapy for Tissue Regeneration01:21

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Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
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Implantation of Ferumoxides Labeled Human Mesenchymal Stem Cells in Cartilage Defects
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Mesenchymal Stem Cell Engineering.

Shuang Liu1

  • 1Department of Pharmacology, Ehime University Graduate School of Medicine, Toon, Ehime, Japan. liussmzk@m.ehime-u.ac.jp.

Methods in Molecular Biology (Clifton, N.J.)
|January 25, 2024
PubMed
Summary
This summary is machine-generated.

Mesenchymal stem cells (MSC) show potential for cartilage repair and treating arthritis. Gene-modified MSC using CRISPR technology offer a promising therapeutic strategy for regenerating diseased joints, particularly in rheumatoid arthritis (RA).

Keywords:
CRISPR/Cas9Genomic manipulationMesenchymal stem cellsPuromycin selectionRegenerative potential

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Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Mesenchymal stem cells (MSC) are multipotent cells capable of tissue regeneration.
  • MSC applications include cartilage repair for chondral/osteochondral lesions and supporting joint repair in arthritis.
  • Enhancing MSC chondrogenesis via gene therapy is a key area for improving regenerative capacity.

Purpose of the Study:

  • To explore the potential of gene-modified MSC for enhanced chondrogenesis.
  • To investigate the therapeutic application of modified MSC in joint regeneration for conditions like rheumatoid arthritis (RA).

Main Methods:

  • Utilized clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) system for genomic manipulation of MSC.
  • Investigated the chondrogenic potential of gene-modified MSC.

Main Results:

  • Gene modification of MSC using CRISPR/Cas technology shows promise for enhancing chondrogenesis.
  • Targeted gene-modified MSC represent a potential therapeutic avenue for joint diseases.

Conclusions:

  • Gene-modified MSC hold significant potential for the regenerative treatment of diseased joints.
  • CRISPR-based gene editing offers a novel approach to enhance MSC therapeutic efficacy in conditions such as RA.