Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective Enrichment of Non-Canonical Vγ9-Jγ2 TRG Clonotypes in Clear Cell Renal Cell Carcinoma With Shorter CDR3 Loops.

Immune network·2026
Same author

Multi-Model Machine Learning for Survival Predictions for Castration-Resistant Prostate Cancer.

Cancers·2026
Same author

Predictive value of primary tumour SUV<sub>max</sub> on <sup>18</sup>F-fluoro-2-deoxy-D-glucose positron emission tomography/computed tomography in patients undergoing fertility-sparing treatment for early endometrial cancer.

European journal of obstetrics, gynecology, and reproductive biology·2026
Same author

RNA-DNA Fusomer Fibers With Customizable Physicochemical, Mechanical, and Biological Properties for Next-Generation Therapeutics.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

The Influence of Calcination Temperature on Metal Tungstate Properties and Photodegradation.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Increased Graft Diameter and Quadrupled Versus Tripled Hamstring Tendon Autograft Are Associated With Improved Graft Survival in Double-Bundle Anterior Cruciate Ligament Reconstruction.

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association·2026

Related Experiment Video

Updated: Jul 5, 2025

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

15.9K

Design and Characterization of Compact, Programmable, Multistranded Nonimmunostimulatory Nucleic Acid Nanoparticles

Ross Brumett1, Leyla Danai2, Abigail Coffman1

  • 1Department of Chemistry, Ball State University, Muncie, Indiana 47306, United States.

Biochemistry
|January 25, 2024
PubMed
Summary

Single-stranded thymidine (ssT) linkers enhance DNA nanostructure flexibility without compromising stability. This finding enables the design of versatile, stable, and non-toxic DNA nanoparticles for biomedical applications.

More Related Videos

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.5K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.5K

Related Experiment Videos

Last Updated: Jul 5, 2025

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
08:51

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

Published on: March 1, 2013

15.9K
Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
09:09

Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery

Published on: May 2, 2019

7.5K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.5K

Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • DNA nanostructures offer versatile platforms for various applications.
  • Understanding the factors influencing their stability and flexibility is crucial for rational design.

Purpose of the Study:

  • To investigate the role of single-stranded thymidine (ssT) linkers in DNA nanostructure stability and flexibility.
  • To optimize the design of DNA nanostructures for enhanced performance and biomedical applications.

Main Methods:

  • Systematic variation of ssT linker lengths in three-way junction (3WJ) motifs.
  • UV melting experiments and molecular dynamics simulations to assess stability and flexibility.
  • Assembly and characterization of triangular DNA nanoparticles with varying ssT lengths.

Main Results:

  • Increasing ssT regions minimally impacted thermodynamic stability but significantly enhanced 3WJ flexibility.
  • Triangular DNA nanoparticles exhibited efficient assembly (except for 0T), enhanced serum stability, and were non-toxic.
  • The 4T 3WJ was identified as a versatile building block due to its flexibility and favorable properties.

Conclusions:

  • ssT linkers are key modulators of DNA nanostructure flexibility, crucial for designing adaptable nanosystems.
  • Optimized DNA nanostructures possess enhanced stability, biocompatibility, and potential for cost-effective production.
  • Findings pave the way for advanced nucleic acid nanotechnology in biomedical fields.