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Rapid autoxidation of ferrous heme-Aβ complexes relevant to Alzheimer's disease
Madhuparna Roy1, Abhishek Dey1, Somdatta Ghosh Dey1
1Indian Association for the Cultivation of Science, School of Chemical Sciences, 2A & 2B, Raja S. C. Mullick Road, Jadavpur, Kolkata 700032, India. icsgd@iacs.res.in.
Abstract:
Heme bound Aβ peptides have been reported to reduce O2 by 2e- to H2O2 which may result in oxidative stress commonly encountered in Alzheimer's disease. In this study we report the first instance of rapid freeze quench trapping and characterizing the heme(III)-O2˙- intermediate involved in the heme-Aβ induced formation of partially reduced oxygen species (PROS) in physiologically relevant aqueous medium using absorption and resonance Raman spectroscopy. The kinetics of this process indicates a key role of the Tyr10 residue, unique to human Aβ, in the generation of H2O2 from O2.
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