Aptasensors Based on Non-Enzymatic Peroxidase Mimics: Current Progress and Challenges

Area of Science:

• Biotechnology
• Biosensor Technology
• Analytical Chemistry

Background:

• Traditional immunoassays use antibodies and enzymes, but face challenges like high costs, instability, and variability.
• Oligonucleotide aptamers offer specific, high-affinity binding, superior stability, and cost-effective synthesis compared to antibodies.
• Non-protein peroxidase mimics present a viable alternative to enzymatic signal generation.

Purpose of the Study:

• To review and analyze aptamer-based biosensors (aptasensors) utilizing non-protein peroxidase mimics.
• To explore the potential of these advanced biosensors as alternatives in diagnostics and analysis.

Main Methods:

• Review of literature on aptasensors employing optical detection (colorimetric, luminescent, fluorescent).
• Analysis of biosensors incorporating DNAzymes, nanoparticles, and metal-organic frameworks as peroxidase mimics.

Main Results:

• Aptasensors with peroxidase mimics demonstrate significant potential for sensitive and selective analyte detection.
• These systems overcome limitations of traditional antibody-enzyme based assays.

Conclusions:

• Aptasensors integrating aptamers and peroxidase mimics offer a promising platform for advanced analytical and diagnostic tools.
• This approach enhances stability, cost-efficiency, and reproducibility in biosensing.