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Anti-Proliferative and Apoptotic Activities of Rumex crispus
Sepideh Mohammadhosseinpour1,2, Mukund Bhandari1,3, Dallas A Lee1,4
1Department of Biotechnology, Stephen F. Austin State University, The UT System, Nacogdoches, TX 75965, USA.
Abstract:
Colorectal cancer is the fourth leading cause of cancer death and the third most common cancer diagnosed in the United States. Several anticancer compounds from natural products have been of great interest in cancer chemotherapy and are currently in clinical trials. Natural products that present the targeted killing of cancerous cell and are soluble in water with minimal side effects are ideal candidates. In this study, water-soluble compounds from Rumex crispus plants were screened for anti-proliferative and apoptotic activity against human colorectal adenocarcinoma (DLD-1) cells. The most potent fraction with the highest cell killing and caspase fold change rates was selected for further experiments. The observed changes were further validated by measuring the caspase fold change using RT-qPCR. Furthermore, gene transcript levels were evaluated using an RT2 Profiler assay and a microarray experiment. Our results showed that the most potent L19 fraction exhibits anti-proliferative activity in a dose-dependent manner. The L19 fraction was found to induce apoptotic pathways by triggering different caspases and inflammatory pathways through the activation of non-apoptotic genes. Our study identified and validated the anticancer property of the L19 fraction, which can serve as a strong lead compound for the synthesis of other novel potent analogues.
Insights
Researchers explored natural compounds for colorectal cancer treatment. A water-soluble fraction from Rumex crispus (L19) demonstrated significant anti-proliferative and apoptotic effects on cancer cells, showing promise as a novel therapeutic lead.
Area of Science:
- Natural Product Chemistry
- Cancer Biology
- Pharmacology
Background:
- Colorectal cancer is a leading cause of cancer death globally.
- Natural products offer potential anticancer agents with targeted efficacy and fewer side effects.
- Water-soluble compounds are desirable for chemotherapy development.
Purpose of the Study:
- To screen water-soluble compounds from Rumex crispus for anti-proliferative and apoptotic activity against human colorectal adenocarcinoma (DLD-1) cells.
- To identify and validate a potent anticancer fraction for further development.
- To investigate the molecular mechanisms underlying the observed anticancer effects.
Main Methods:
- Screening of water-soluble Rumex crispus fractions against DLD-1 cells.
- Dose-dependent anti-proliferative assays.
- Caspase activity measurement via RT-qPCR.
- Gene expression analysis using RT2 Profiler assay and microarray.
Main Results:
- The L19 fraction of Rumex crispus exhibited significant dose-dependent anti-proliferative activity against DLD-1 cells.
- The L19 fraction induced apoptosis by activating caspases and inflammatory pathways.
- Gene expression analysis revealed activation of non-apoptotic genes contributing to the anticancer effect.
Conclusions:
- The L19 fraction from Rumex crispus possesses validated anticancer properties.
- This fraction acts through induction of apoptotic and inflammatory pathways.
- The L19 fraction is a promising lead compound for developing novel colorectal cancer therapeutics.
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