Related Experiment Video
Updated: Jul 4, 2025
08:28
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture
Published on: May 26, 2016
16.3K
Plant Growth Regulation in Cell and Tissue Culture In Vitro
Taras P Pasternak1, Douglas Steinmacher2
1Instituto de Bioingeniería, Universidad Miguel Hernández, 03202 Elche, Spain.
Plants (Basel, Switzerland)
|January 26, 2024
Summary
This review details key factors for successful plant tissue culture and in vitro regeneration, offering practical insights for plant biotechnologists to develop more resilient crops.
Area of Science:
- Plant Biotechnology
- Plant Physiology
- Cell Biology
Background:
- Plant tissue culture and in vitro regeneration are vital for developing productive and resilient plants.
- Current techniques often rely on empirical studies, with limited data-driven models.
- Optimizing plant regeneration requires understanding numerous controlling factors.
Purpose of the Study:
- To provide a comprehensive overview of critical factors influencing plant tissue culture success.
- To offer practical recommendations for plant biotechnologists based on extensive research experience.
- To enhance the impact and application of plant tissue culture in industry.
Main Methods:
- Review of existing scientific literature on plant physiology and tissue culture.
- Synthesis of data-driven models and empirical findings.
- Incorporation of over 20 years of practical research experience.
Main Results:
- Identified key factors: nutrient availability, endogenous auxin synthesis, organic compounds, and environmental conditions.
- Detailed analysis of each factor's role in plant tissue culture and regeneration.
- Provided practical recommendations for optimizing protocols.
Conclusions:
- A thorough understanding of controlling factors is essential for advancing plant biotechnology.
- This review serves as a guide for new researchers and industry professionals.
- Improved plant tissue culture techniques will contribute to more resilient and productive agriculture.
More Related Videos
08:50In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines
Published on: April 18, 2025
404
11:01Nutrient Regulation by Continuous Feeding for Large-scale Expansion of Mammalian Cells in Spheroids
Published on: September 25, 2016
7.7K
Related Concept Videos
01:21Cell Culture
17.2K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
17.2K
02:57Plant Tissue Culture
37.9K
Plant tissue culture is widely used in both primary and applied science. Applications range from plant development studies to functional gene studies, crop improvement, commercial micropropagation, virus elimination, and conservation of rare species.
37.9K
01:17Stem Cell Culture
5.2K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.2K
02:19Morphogenesis
28.2K
Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.
28.2K
02:36Cells Coordinate Growth and Proliferation
4.5K
Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...
4.5K
02:50Transgenic Plants
7.2K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.2K