Related Experiment Video
Updated: Jul 4, 2025
10:53
Concentration of Virus Particles from Environmental Water and Wastewater Samples Using Skimmed Milk Flocculation and Ultrafiltration
Published on: March 17, 2023
1.6K
Sample Processing and Concentration Methods for Viruses from Foods and the Environment Prior to Detection
Sloane Stoufer1, Anand R Soorneedi1, Minji Kim1
1Department of Food Science, University of Massachusetts, Amherst, Massachusetts, USA;
Annual Review of Food Science and Technology
|January 26, 2024
Summary
Concentrating viruses from food samples is crucial for detecting low-level contamination. This review surveys various methods, discussing their pros, cons, and emerging techniques for effective foodborne virus detection.
Area of Science:
- Food safety and virology
- Microbiology and public health
Background:
- Viruses are a primary cause of global foodborne illnesses.
- Effective detection of foodborne viruses necessitates concentration due to low contamination levels in food matrices.
Purpose of the Study:
- To review and compare various foodborne virus concentration techniques.
- To discuss the efficacy of these methods across different food and environmental samples.
- To explore emerging purification and concentration technologies for viral pathogens.
Main Methods:
- Classification of virus concentration methods into nonspecific and specific approaches.
- Evaluation of techniques based on physicochemical properties or antibody-based capture.
- Analysis of factors including cost, time, usability, and inhibitor removal.
Main Results:
- Numerous nonspecific and specific virus concentration techniques exist, each with unique advantages and limitations.
- Method selection depends on factors such as cost, speed, ease of use, and matrix interference.
- Emerging techniques offer potential advancements in purification and concentration.
Conclusions:
- Appropriate selection of virus concentration methods is vital for accurate foodborne virus detection.
- Ongoing research into novel techniques aims to improve efficiency and reliability in food safety testing.
- Understanding method limitations is key to mitigating risks associated with viral contamination in food.
