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Updated: Jul 4, 2025

Synchronization of Caulobacter Crescentus for Investigation of the Bacterial Cell Cycle
Published on: April 8, 2015
Cell cycle-coordinated maintenance of the Vibrio bipartite genome
Théophile Niault1,2, Jakub Czarnecki1, Morgan Lambérioux1,2
1Bacterial Genome Plasticity Unit, CNRS UMR3525, Institut Pasteur, Université Paris Cité , Paris, France.
Abstract:
To preserve the integrity of their genome, bacteria rely on several genome maintenance mechanisms that are co-ordinated with the cell cycle. All members of the Vibrio family have a bipartite genome consisting of a primary chromosome (Chr1) homologous to the single chromosome of other bacteria such as Escherichia coli and a secondary chromosome (Chr2) acquired by a common ancestor as a plasmid. In this review, we present our current understanding of genome maintenance in Vibrio cholerae, which is the best-studied model for bacteria with multi-partite genomes. After a brief overview on the diversity of Vibrio genomic architecture, we describe the specific, common, and co-ordinated mechanisms that control the replication and segregation of the two chromosomes of V. cholerae. Particular attention is given to the unique checkpoint mechanism that synchronizes Chr1 and Chr2 replication.
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